Translational_Unit

Part:BBa_K4310010:Design

Designed by: Seungchan Oh   Group: iGEM22_UIncheon   (2022-10-11)


constitutively expressed eGFP


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 1171
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 1171
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 226
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 1171
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 1171
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1398
    Illegal BsaI.rc site found at 1677
    Illegal SapI site found at 437
    Illegal SapI site found at 484
    Illegal SapI.rc site found at 471
    Illegal SapI.rc site found at 1216


Design Notes

To make compatible to our assembly, CAMV 35S and Tnos are amplified by overhang primers.


Source

CAMV 35S: BBa_J428074 from distribution 2022 Kozak sequence: BBa_K4310003 eGFP: BBa_K431006 Tnos: BBa_J428082 from distribution 2022

References